You are studying bacteria in a lab setting. You want to know…
You are studying bacteria in a lab setting. You want to know if your bacterium is able to use citrate as a carbon source and ammonium as a nitrogen source. To complete this test you use citrate agar, which contains 5g NaCl, 2g Sodium citrate, 1g Ammonium dihydrogen phosphate, 1g diphosphate, 15g Agag, and 0.08g bromothymol blue. Bromothymol blue is a pH indicator. If a bacterium is able to grow and use the citrate and ammonium salts the pH increases and the media turns blue. How is this media described?
Read DetailsYou are preforming an assay in the lab where you want to det…
You are preforming an assay in the lab where you want to determine the antibiotic sensitivity for a bacterium. You have three cultures of this bacterium that you can choose from to perform the antibiotic sensitivity assay. Culture 1 is a freshly inoculated broth; culture 2 is a culture that has been growing for 6 weeks; culture 3 has been growing for 12 hours and is slightly turbid. Use your knowledge of the bacterial growth curve to determine which culture you should use in your assay and explain why this one was chosen.
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