An 8-residue polypeptide was determined to have the followin…
An 8-residue polypeptide was determined to have the following amino acid composition: (Met)2, Phe, Pro, Gln, Arg, Gly, Tyr. The first-round of Edman degradation released PTH-Gly. Both trypsin treatment and CNBr treatment yielded a tripeptide and a pentapeptide, respectively (with different sequences). Digestion with chymotrypsin (note: only consider cleavage next to aromatic amino acids) produced a tetrapeptide, a tripeptide and a free methionine. The amino acid sequence of this octapeptide should be:
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For question 3, choose to respond to ONE of the following prompts: 3A) Phyllis Wheatley’s “To the University of Cambridge in New England” addresses students at a prestigious institution. How does Wheatley’s appeal to the value of education and virtue reflect ideals important to the new American republic? And, how does Wheatley establish her own authority in speaking—particularly given her identity that is so significantly different from that of her audience? 3B) Benjamin Franklin’s writings often emphasize self-improvement and civic responsibility as central to American identity. How does “The Way to Wealth” promote these ideals, and how does it reflect an emerging culture of the American colonies that differs from earlier writers ? (For this response, you need to discuss “The Way to Wealth,” but you can also reference our in-class discussion of Franklin’s Autobiography or his other works.)
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