!! SPOILER ALERT !! The following questions contain spoilers…
!! SPOILER ALERT !! The following questions contain spoilers of the film. You are advised to read them after you have finished the film so as not to spoil the ending. If you’ve already seen it…go on right ahead and read them. Choose at least TWO of the following questions to answer. In your responses, make sure you number your answer to correspond to the question number you are addressing. At the end of the final battle between T’Challa and Killmonger–after T’Challa offers to save his life and be under the supervision of Wakanda–Killmonger says “Bury me in the ocean, with my ancestors that jumped from the ships, because they knew death was better than bondage.” This powerful moment
Read DetailsYou are performing a lineage tracing experiment to test whet…
You are performing a lineage tracing experiment to test whether any liver cells can migrate away form the liver organ. You decide to implement a genetic lineage tracing strategy (outlined below) to track liver cells throughout the life of your study organism. You establish two separate transgenic lines Line 1 and Line 2. Line1 has a transgene that drives Cre recombinase under the control of a strong liver enhancer plus promoter. Line 2 features a transgene that drives a green fluorescent protein (GFP) with a stop codon followed by a red fluorescent protein (RFP) gene under the control of a housekeeping enhancer plus promoter. The GFP portion of this transgene (including the stop codon) is flanked by LoxP sites, which upon Cre exposure, cause removal of the GFP coding region and leave the RFP coding region in-frame. Line 1: Line 2: Before we cross Line1 and Line2 together… we expect Line1 to demonstrate [blank1] fluorescence by itself. we expect Line2 to demonstrate [blank2] fluorescence by itself. After crossing Line1 and Line2 together… we expect brain cells to demonstrate [blank3] fluorescence. we expect liver cells to demonstrate [blank4] fluorescence. if you find cells that demonstrate red fluorescence outside of the liver, this would [blank5] your hypothesis.
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