This image is from the BAP of a sputum culture. The sample was collected via leukens tube from a 72 year-old patient in the ICU. There was no growth on the MAC and the growth on the CHOC plate resembles the BAP. What is the next step in the work-up?

Examine the broth microdilution susceptibility test shown above and determine the MIC for gentamicin.

A patient with chronic obstructive pulmonary disease has been admitted to the hospital with a possible pneumonia. Direct Gram stain of the sputum reveals many neutrophils, few squamous cells, a few Gram positive cocci in pairs, clusters and short chains, and numerous plump Gram negative diplococci. The culture grows upper respiratory flora and many gray-white opaque smooth colonies. When the laboratory scientist attempts to work with one of the colonies, it moves on the surface of the agar without breaking apart. Further testing reveals the following results: Glucose = negative Nitrate = positive Maltose = negative DNase = positive Lactose = negative Oxidase = positive Sucrose = negative Catalase = positive The most likely causative agent for this patient’s pneumonia is:

Kirby-Bauer susceptibility testing requires: 1. organisms in suspension with a turbidity equal to a 5.0 McFarland standard. 2. organisms with a rapid rate of growth. 3. standardized depth of Mueller-Hinton agar. 4. incubation in increased CO2 incubator.

Both sets of blood cultures from a 44 year-old male patient (post appendectomy) grew Gram negative bacilli from both the aerobic and anaerobic bottles after 18 hours incubation. Biochemical reactions demonstrate the following: MAC = pink donuts BAP = luxuriant growth TSI = acid / H2S Indole = negative Motility = positive Citrate = positive MR/VP = +/0 Ornithine = negative Lysine decarboxylase = negative PAD = negative The most likely genus and species of this organism is:

The Method acting style developed from what other acting style?

A catalase positive organism is growing on a mannitol salt agar plate and it turns bright yellow. What is the most likely identification of this organism?

A Gram negative bacillus was isolated from the CSF of an infant in the neonatal ICU. The infant was born 18 days ago at an estimated gestational age of 27 weeks. Biochemical reactions demonstrate the following: TSI = acid/acid no H2S Lysine decarboxylase = purple Indole = positive Arginine dihydrolase = yellow Citrate = peacock blue Ornithine decarboxylase = yellow MR/VP = 0/+ Decarboxylase base = yellow Urea = Magenta pink PAD = Negative Motility = Negative The most likely genus and species of this organism is:

True of False, all acting theories are a search for “The Truth.”

These images are from the culture of an expectorated sputum specimen from a 67 year old patient suffering from symptoms of pneumonia (BAP on the left, CHOC on the right, MAC showed no growth). What are the next steps in working up this culture?