A primer used in dideoxy DNA sequencing is 20 nucleotides long and has the following sequence:  5’-GGATCCATGACTAGTCCGAC-3’. A segment of DNA is cloned into a vector and then the vector DNA is denatured and subjected to dideoxy DNA sequencing method. Below is the DNA sequence from a region of the vector. The primer-annealing site is shown in bold and underlined.    3-CCCGATCGGCCTAGGTACTGATCAGGCTGAATGACT-5’   Based on the sequence above, what would be the size(s) of the band(s) (i.e., the number of nucleotides in each band) in which dideoxyT had been added to the sequencing reaction?

Work in a forensic lab and you need to do a paternity test. You would:

You observe a small patch of white hair on the head of an infant. The possible genetic explaination for this is:

You can use tandem mass spectrometry to:

You want to do an experiment to check after application of a hormone to cells a protein is has multiple phosphate groups attached to it. You would use a:

The identification of a stop codon for a particular gene is an example of

The Cas9 protein in CRISPR-Cas technology will:

Positional cloning does not use

Below are various steps in the cloning process. Which is the correct order?  1. Transform vector into bacteria. 2. Cut the vector and chromosomal DNA with the same restriction enzyme. 3. Add DNA ligase. 4. Plate on growth media containing an antibiotic such as ampicillin. 

The restriction enzyme EcoRI recognizes the sequence: 5′-G/AATTC-3′ 3′-CTTAA/G-5′ It cuts between the G and A as noted by the slashes. Following the digestion of chromosomal DNA and vector DNA with EcoRI, let’s suppose that one fragment of chromosomal DNA gets inserted into a vector. No DNA ligase has been added. If both of the sticky ends of the chromosomal DNA fragment are binding to the sticky ends of the vector, what is holding these two pieces of DNA together?