You recreated experiments studying the lac operon in E. coli (see schematic below). In your experiments, the activity of the enzyme β-galactosidase is visibly measured by addition of X- gal and IPTG into the growth media (lactose is present as well). X-gal is a lactose analog that turns blue when metabolized by β-galactosidase, but it does not induce the lac operon. IPTG is another analog of lactose that is an inducer of the lac operon but is not metabolized by enzyme β-galactosidase. In your experiment an E. coli without a functional “lac i” gene would be expected to ________.

Answer the next four questions using the following sequence and Codon table ABOVE. Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a short hypothetical protein. Both strands are shown; the top strand reads 5’ to 3’ left to right, while the bottom strand reads 5’ to 3’ right to left. The nucleotides are numbered 1 to 100. Transcription begins with and includes the bold and underlined C/G (top strand/bottom strand) at position 9.     Transcribe this gene into mRNA. What are the first 10 nucleotides of the resulting mRNA transcribed from this gene?

Answer the next three questions using the following sequence and Codon table ABOVE. Shown below is a double-stranded bacterial (E. coli) DNA sequence coding for a short hypothetical protein. Both strands are shown; the top strand reads 5’ to 3’ left to right, while the bottom strand reads 5’ to 3’ right to left. The nucleotides are numbered 1 to 100. Transcription begins with and includes the bold and underlined C/G (top strand/bottom strand) at position 9. Translate this gene (hint: use mRNA from your transcription). What are the first 5 amino acids of the resulting polypeptide translated from this gene?

Which of the following mutations would be most likely to have the most harmful effect on an organism?

Amanatin, a toxin from the death cap poisonous mushroom, inhibits RNA polymerase, so it

The nurse is performing an initial assessment on an ambulatory and independent 25 year old female patient and notes that the patient weighs 186.4 lb and is 5 foot 8 inches tall (BMI 28.3). Three months ago, the patient reported she weighed 212 lb. The patient reports that they have had moderate to severe fatigue, moderate anorexia, and multiple episodes of intermittent steattorhea and bloating over the last month. The patient is worried she may have Crohn’s disease because she has a cousin with the disorder and reports that she has been feeling anxious about the possible diagnosis but doesn’t report any symptoms of depression. Using the Mini Nutritional Assessment (below) the nurse identifies the patient [score] for malnutrition and decides to [plan].

The nurse is preparing to administer nitroglycerin at a rate of 0.5 mcg/kg/min for a patient weighing 154 lbs. You have a nitroglycerin premixed bottle (see image below).What rate in mL per hour should the nurse set on the infusion pump?Round to the nearest tenth. The answer must be numeric only. Don’t add any units of measurement.

Which age range is associated with germ-cell tumors?

Normal ovarian arterial flow can be described best as:

Which germ-cell tumor is associated with an increased AFP and has an extremely poor prognosis?