In a direct ELISA performed in lab, the [item1] is alread…
In a direct ELISA performed in lab, the [item1] is already adsorbed to the bottom of the wells before the experiment begins. In the next step, [item2] is added to each well followed by triple wash . In the next step, [item3] is added to attach to the potentially present antibody from patient fluid. In the last step, [item4] is added and a color change will occur in a positive sample.
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