In a laboratory experiment using spectrophotometry, an enzym…
In a laboratory experiment using spectrophotometry, an enzyme is combined with its substrate at time zero. The absorbance of the resulting solution is measured at time zero and at five-minute intervals. In this procedure, an increase in absorbance is related to the amount of product formed during the reaction. The experiment is conducted using the three preparations shown in the table below. Absorbance of Solution at different times Enzyme Preparation 0 min 5 min 10 min 15 min 20 min I. 3 mL of enzyme preparation 2 mL of substrate pH 5.0 0.0 0.22 0.33 0.38 0.37 II. 3 mL of boiled enzyme preparation 2 mL of substrate pH 5.0 0.0 0.06 0.04 0.03 0.04 If you were asked to graph this data, where would you place the dependent variable on the graph?
Read DetailsTo study the actions of the enzyme catalase on hydrogen pero…
To study the actions of the enzyme catalase on hydrogen peroxide, students performed the following experiment. Catalase was extracted from potatoes by blending raw potatoes in a blender with cold distilled water. The filtrate was stored on ice. The following hydrogen peroxide solutions were made: 1 %, 5%, 10%, and 15%. Filter paper disks were soaked in the catalase filtrate and dropped into beakers containing the various solutions. The activity of the enzyme was measured by the amount of time it took for the disks to float to the surface of the solution on the bubbles produced by the reaction. The following data were obtained. Time it takes for disks to float in different Hydrogen Peroxide concentrations Hydrogen Peroxide Solution Average Time, in seconds, for disks to float 1% 30 sec 5% 25 sec 10% 20 sec 15% 10 sec Which of the following experimental designs should the students use as a control for the experiment?
Read DetailsA sample of human blood was placed in a test tube containing…
A sample of human blood was placed in a test tube containing a physiological saline solution (0.9% sodium chloride). This type of solution is often used intravenously to quickly rehydrate patients. A drop of the blood from the test tube was placed on a slide and red blood cells (RBCs) were observed under a microscope. Three possible outcomes are diagrammed below. Which of the following best predicts which diagrammed microscope view the laboratory worker would see and best explains why?
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