GradePack

    • Home
    • Blog
Skip to content
bg
bg
bg
bg

GradePack

The seating section of the main body of the church; one of t…

The seating section of the main body of the church; one of two main areas of the church, the other being the chancel is called the _______________________

Read Details

The founder of the mormon church; and translater of book of…

The founder of the mormon church; and translater of book of mormon

Read Details

Isoelectric focusing gel electrophoresis separates non-denat…

Isoelectric focusing gel electrophoresis separates non-denatured proteins and peptides by their isoelectric points using a pH gradient gel. Would you predict that this peptide would stop its travel through the gel in the acidic or basic portion of the gel? Briefly justify your choice.

Read Details

In the following procedure, the protein of interest, CA, is…

In the following procedure, the protein of interest, CA, is initially concentrated by precipitation from a 30% saturated ammonium sulfate solution. If you were interested in using ammonium sulfate precipitation to isolate a highly charged peptide, would you expect the peptide to precipitate from a low or high concentration ammonium sulfate in solution?  Briefly justify your answer.   Procedure from the reference cited below: “Briefly, cells expressing the wild-type CA protein were lysedthrough a microfluidizer. Soluble CA protein in the clarifiedlysate was concentrated by precipitation from 30% saturatedammonium sulfate. CA protein was redissolved in 50 mM Tris(pH 8.0) and functionally purified by the addition of sodiumchloride to a final concentration of 2.5 M. After two rounds offunctional purification, the wild-type CA protein wasresuspended in 50 mM sodium phosphate buffer (pH 7.5)and dialyzed against the same buffer. The dialyzed sample wasfurther purified by a subtractive anion exchange chromatography step using a Q-HP HiTrap column (catalog no. 17-1154-01, GE Healthcare, Piscataway, NJ). Purification of 2Mut and 4Mut CA mutants followed thesame protocol that was used for wild-type CA except that200 mM β-mercaptoethanol was included in all buffersthroughout the process. The functionally purified capsidmutant proteins were redissolved in 50 mM Tris (pH 7.5) and40 mM β-mercaptoethanol prior to dialysis in the same buffer,and a subsequent purification with subtractive anion exchangechromatography was performed.” A Trimer of Dimers Is the Basic Building Block for Human Immunodeficiency Virus-1 Capsid Assembly.

Read Details

An auditor selected items for test counts from the client’s…

An auditor selected items for test counts from the client’s warehouse during the physical inventory observation. The auditor then traced these test counts into the detailed inventory listing that ultimately agreed to the financial statements. This procedure most likely provided evidence concerning management’s assertion of

Read Details

Glycine is acetic acid (ethanoic acid) with an amine group a…

Glycine is acetic acid (ethanoic acid) with an amine group attached i.e. 2-amino acetic acid. The pKa of the glycine carboxyl group is 2.34 and the pKa of acetic acid is 4.75.  Briefly explain why there is such a large difference in the pKa values between these two carboxylic acid groups.

Read Details

A paragraph in the Opinion Section of the auditors’ report s…

A paragraph in the Opinion Section of the auditors’ report states: “In our opinion, with the exception of the effects of not observing inventory in one of the client’s Siberian warehouses, … the financial statements present fairly, in all material respects…” This paragraph expresses a(n)

Read Details

A prayer recited at the bedside after death has occurred is…

A prayer recited at the bedside after death has occurred is called __________________________________.

Read Details

How many residues are there in the sequence with acidic side…

How many residues are there in the sequence with acidic side chains (R groups) that have a pKa of 5 or less? Identify these amino acids by name or acceptable abbreviation.

Read Details

Which of the following activities is ordinarily performed pr…

Which of the following activities is ordinarily performed prior to the date of the financial statements?

Read Details

Posts pagination

Newer posts 1 … 37,443 37,444 37,445 37,446 37,447 … 76,641 Older posts

GradePack

  • Privacy Policy
  • Terms of Service
Top