Below are various steps in the cloning process. Which is the correct order?  1. Transform vector into bacteria. 2. Cut the vector and chromosomal DNA with the same restriction enzyme. 3. Add DNA ligase. 4. Plate on growth media containing an antibiotic such as ampicillin. 

The restriction enzyme EcoRI recognizes the sequence: 5′-G/AATTC-3′ 3′-CTTAA/G-5′ It cuts between the G and A as noted by the slashes. Following the digestion of chromosomal DNA and vector DNA with EcoRI, let’s suppose that one fragment of chromosomal DNA gets inserted into a vector. No DNA ligase has been added. If both of the sticky ends of the chromosomal DNA fragment are binding to the sticky ends of the vector, what is holding these two pieces of DNA together?

Which of the following events occur in a single cycle of PCR?

A Southern Blot is used to detect:

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Under the Tokugawa Shogunate, controlling the Daimyo was a priority.  What was one way the Shogun ensured a Daimyo would not rise up against him?

During WWI, Japan sought to expand economically.  What did they do?

The nurse assesses the older adult noting the presence of arcus senilis. Which of the following assessment findings would support this condition?

The nurse is assessing the client who is newly admitted to the nursing home. The client has a history of falls, and is a 1 assist with transfers and ambulation. Which of the following findings would alert the nurse to take action? (Select all that apply).

The nurse knows teaching regarding vision health has been effective when the client states which of the following?