Briefly explain why the streak plate method is better for generating pure cultures than using broth to dilute microbes.  (2 points)

Your patient has impetigo, a skin infection caused by Staphylococcus aureus.  You test bacteria grown from fluid from the lesions for antibiotic sensitivity. Impetigo lesions What is an antibiotic, tested here, that could be used to treat the patient’s infection, effectively? a. ampicillinb. Bactrimc. streptomycin [antibiotic] What is the name of the enzyme that bacteria use to degrade penicillin and other antibiotics of its class? a. β-lactamaseb. hexose kinasec. glucose-6-phosphate dehydrogenased. RuBisCo [enzyme] Among the antibiotics tested listed, above, which one works by inhibiting a metabolic/biochemical pathway? a. aminoglycosideb. beta-lactamc. macrolided. sulfonamide (eg., Bactrim) [metabolic] What is the most common mechanism of resistance to sulfonamide antibiotics such as Bactrim? [Bactrim] a.  beta-lactamase b.  bypass c.  efflux pumps d.  carbapenimase  

Your patient who’s on a ventilator has pneumonia caused by Pseudomonas aeruginosa.  You have tested bacteria cultured from his pleural effusions (lung fluid) for antibiotic sensitivity. What is an antibiotic tested here that could be used to treat the patient’s infection? a. ampicillinb. tetracyclinec. Bactrimd. streptomycin [antibiotic] What is the mechanism of action of ampicillin/penicillin? a. inhibit cell wall formationb. inhibit protein synthesisc. inhibit cell membrane functiond. inhibit metabolism [mechanism] Why are Pseudomonas bacteria resistant to so many antibiotics?  [resistant] a.  They bypass the metabolic pathways affected by the antibiotics. b.  They have a number of efflux pumps. c.  They are uncommonly sensitive to mutation. d.  They produce beta-lactamase, naturally.

You are working with Doctors Without Borders in Polynesia, and meet with a patient who has grossly swollen feet and legs (see below).  (3 points, total) A smear of the patient’s blood is shown, below. What is the name of the disease that is causing your patient’s distress?  [disease] a.  bilharzia b.  dracunculiasis c.  elephantiasis d.  trichinellosis What is the proper name (Genus + specific epithet) of the pathogen that is causing the disease?  [propername] a.  Schistosoma mansoni b.  Dracunculus medininensis c.  Wuchereria bancrofti d.  Trichinella spiralis How is this pathogen transmitted to humans?  [transmission] a.  larvae in water b.  ingestion of copepods c.  mosquito bite d.  undercooked pork

You are working with a patient in the community clinic who complains of a sore throat (see below for a picture of the patient’s throat).  You submit throat swabs to the clinical lab for culturing and identification.  See below for results of lab tests. Growth on blood agar – note clear zone around colonies Gram stain – note chains of purple cocci Catalase test – no bubbles What is the proper name of the bacterium that is the most likely cause of the patient’s distress? a. Staphylococcus aureusb. Staphylococcus epidermidisc. Streptococcus pneumoniaed. Streptococcus pyogenes [propername] What do we mean when we say “beta-hemolysis”? a. lysis of red blood cells with incomplete degradation of hemoglobinb. lysis of red blood cells with no degradation of hemoglobinc. lysis of red blood cells with complete degradation of hemoglobind. no lysis of red blood cells or hemoglobin [beta-hemolysis] What do we mean when we say “alpha-hemolysis”? a. lysis of red blood cells with incomplete degradation of hemoglobinb. lysis of red blood cells with no degradation of hemoglobinc. lysis of red blood cells with complete degradation of hemoglobind. no lysis of red blood cells or hemoglobin [alpha-hemolysis]

For much of the 20th century, people who lived in the Southern United States were stereotyped as being slow-witted and lazy.  However, it is believed that much of this behavior was due to infestation with hookworm which can cause severe anemia due to it feeding on the host’s blood.  What is the proper name (Genus and specific epithet) of this parasite?

To prevent heartworm, cats and dogs should receive appropriate prophylactic medication.  What is the proper name  of the parasite responsible for heartworm? [heartworm] a.  Schistosoma mansoni b.  Dirofilaria immitis c.  Arthrobotrys d.  Echinococcus granulosarum To which major group of helminths does the heartworm parasite belong?  [majorgroup] a.  Annelida b.  Cestodes c.  Nematodes d.  Trematodes How is this parasite transmitted?  [transmission] a.  fleas b.  mosquitoes c.  biting flies d.  ticks

Roger is still not eating which appetite stimulant is the veterinarian most likely to prescribe?

Roger is going to be hospitalized for at least for 4 days, how long can his IVC remain in place? IVC is still patent.

The veterinarian asks you to administer a dose of Cyanocobalamin at 25mcg/kg, SC. How many mL and mcg of Cyanocobalamin will you administer to Roger?