The polymerase chain reaction (PCR) is used to generate:

Apply your knowledge of CRISPR-Cas9 gene editing to design a guide RNA to target a specific gene for modification. Which of the following components is essential for CRISPR-Cas9 gene editing?

One of the most significant conflicts in this novel is Junior’s struggle to be an individual and also a member of his tribe. Which do you think is more important: being an individual or a member of the community?  Why? Also, given the novel so far, which direction do you think the author is leaning on this question? Explain using examples from the novel (so far) to back up your claims.

You have a PMHNP co-worker who is new to practice. She asks if you will consult on one of her patients. She inherited a patient from a psychiatrist in town that has since retired. The patient is a 52-year-old male that has a history of bipolar disorder and is currently taking: depakote ER 1000 mg twice daily, lamotrigine 200 mg daily, abilify 30 mg daily, zyprexa 20 mg daily, seroquel 100 mg daily at night and clonazepam 1 mg three times daily. She notes the patient “looked like he was drooling” during the appointment and was concerned with all the medications that he was on and interactions. When looking at the combination of depakote and lamotrigine what is your best response:

Mary is a 17-year-old female that comes to the clinic with concerns of depression. She has been depressed for greater than two years and she has not felt relief from this depression for greater than two months. You diagnose her with persistent depressive disorder and start her on fluoxetine. She presents back to the clinic in one month and she is chipper, much happier and states she has not felt this good in years and has started different projects and feels like she has so much energy she does not need to sleep. She is also wearing generous amounts of makeup and not dressed for the weather. What is something to consider with this presentation: 

A nurse is assessing a client with left-sided heart failure. For which clinical manifestations should the nurse assess? (Select all that apply)   

The nurse outlines behaviors that aid in the prevention of cardiovascular disease, which are: (Select all that apply.)

Jenny is exercising at 45% of her VO2 max. How would increasing the intensity to 60% of her VO2max impact TOTAL fat oxidation?

Review the routing table of R2. How many OSPF routes should be present in the routing table, and why? Please attach a screenshot of R2’s routing table. (2.5 points)

On R1, use the “ip route” command to set up a default route with the IP address of R3’s GigabitEthernet0/0/0 interface as the next-hop. (5 points) On R3, use the “ip route” command to set up a default route with the IP address of R1’s GigabitEthernet0/0/1 interface as the next-hop. (5 points) Examine the routing table of R1. Explain the output, then attach a screenshot of R1’s routing table.  (2.5 points)