If the following question were to be asked during an interview, it would be considered _____: “Do you have the proper paperwork to work in the United States?”

Martha comes in the office and presents a state assistance card, followed by the next patient, Debra, who presents a Blue Cross Blue Shield card. The medical assistant greets Debra with a smile but never acknowledges Martha, and after handing back Martha’s card, the medical assistant makes sure to sanitize her hands. This is an example of

A homozygous individual for a particular gene has:A. Two different alleles for that gene.B. No alleles for that geneC. One allele that is dominant and one that is recessive.D. Two identical alleles for that gene.

At what temperature does the following step of PCR occur?    

You design primers and realize that they fold back on themselves and form structures like the ones shown below. What will you do next?

The following gel represents RNA extracted by four students (1-4)  in a lab. Who was most successful in extracting RNA?

During RNA extraction, you isolate phase A. Your friend repeats the same extraction protocol as you, but isolates phase B. Both of you run the respective phases on the gel to detect RNA.  Which of you is most likely to be successful in RNA isolation? 

During SDS-PAGE electrophoresis, proteins are separated based on size only.

A supercoiled plasmid will run faster compared to its linearized form, because it is more compact.

Protein samples from three patients (1,2 and 3) were separated by SDS-PAGE and subjected to immunoblot analysis.  The blot was incubated with antibodies specific for B-actin and VEGF proteins. Why was the blot probed with antibodies specific for B-actin? Pick all correct answers for complete credit.