What types of tests cannot be performed when someone has outer ear atresia? 

You have just mixed 20 mL of 0.1 M solution of weak acid with 8 mL of 0.1 M solution of NaOH (strong base).  The measured pH is 5.12 for the resulting solution.  Please calculate the pKa value for the weak acid.  

Consider a 9-mer peptide with the sequence Glu-His-Trp-Ser-Gly-Leu-Arg-Pro-Gly and assuming ionization constants of:                        pKCOOH= 2.0, pKNH2= 8.0, pKGlu= 4.0, pKHis= 6.0, pKArg=12.5              

The tense form (T-state) of an allosteric enzyme has higher affinity for the substrates.  

Give the number of charged groups at pH 3.0

The antibiotic penicillin is an example of a drug design known as a “Transition-State Analog Inhibitor”. 

Match the amino acids (using letter in parenthesis) with the appropriate side chain types or properties.

ATP can bind to the regulator subunits of the allosteric enzyme ATCase which catalyzes reaction for the first step of pyrimidine nucleotide biosynthesis.  Which of the following best describes the effect of ATP on ATCase?

An 8-residue polypeptide was determined to have the following amino acid composition: (Met)2, Phe, Pro, Gln, Arg, Gly, Tyr.  The first-round of Edman degradation released PTH-Gly.  Both trypsin treatment and CNBr treatment yielded a tripeptide and a pentapeptide, respectively (with different sequences).  Digestion with chymotrypsin (note: only consider cleavage next to aromatic amino acids) produced a tetrapeptide, a tripeptide and a free methionine.  The amino acid sequence of this octapeptide should be:

For question 3, choose to respond to ONE of the following prompts: 3A) Phyllis Wheatley’s “To the University of Cambridge in New England” addresses students at a prestigious institution. How does Wheatley’s appeal to the value of education and virtue reflect ideals important to the new American republic? And, how does Wheatley establish her own authority in speaking—particularly given her identity that is so significantly different from that of her audience?     3B) Benjamin Franklin’s writings often emphasize self-improvement and civic responsibility as central to American identity. How does “The Way to Wealth” promote these ideals, and how does it reflect an emerging culture of the American colonies that differs from earlier writers ? (For this response, you need to discuss “The Way to Wealth,” but you can also reference our in-class discussion of Franklin’s Autobiography or his other works.)