Patient RBCs tested with commercially prepared Patient plasma tested with commercially prepared Anti-A Anti-B A1 cells B cells 4+ 0 1+ 4+   Analyze and interpret the blood group typing results above for a 36-yr. old male patient. The test has been repeated tat the same results were obtained.  Identify this as a forward or reverse type discrepancy.  Next, indicate the most probable cause for the discrepancy, assuming there are not any technical or clerical errors, and the patient has not been recently transfused.  How would you attempt to resolve this discrepancy to confirm an ABO blood group type? Be specific in you answer.  Provide all test that would be performed as well as the expected results if your initial theory was correct.

The following laboratory results were obtained on a mother and her baby immediately following delivery of the neonate.  Examine the medical history and test results provided below to determine the MOST probable cause of HDFN?                  Mother:           O D Positive Antibody detection/screen results shown below. Cell I.S (Room Temp.) (PEG) AHG IgG-coated RBCs (Pcells) I 0 3+ TNP II 0 3+ TNP III 0 0 P                              TNP: Test Not Performed               Baby:              O D Positive                                     DAT: Positive (2+)                                     Hgb: 11.0 g/dL                                     HCT: 33%                                     Bilirubin: Total 5.6 mg/dL; Direct 1.2 mg/dL

Which of the following makes a unit of RBCs unsuitable to be used for exchange transfusion in a newborn?            

What other antibodies can still not be ruled out after evaluating the entire antibody work-up?

How many units of RBCs are required to raise the hematocrit of a 70-kg, non-bleeding adult from 21% to 30%?

Explain how you know that the correct antibodies have been identified. Include how the “Rule of 3” applies as well as calculated p-values in your explanation.  Students don’t need to show calculation but refer to the p-value in your answer.

Match each blood group system with the appropriate description

Of the statements listed below, which one is the usual rule of thumb that determines when reagent quality control (QC) is performed?

What is the correct storage temperature and expiration date (Shelf life) for Thawed Plasma?

What type of blood (RBCs) would be selected for transfusion?  (Be sure to include ABO and D compatibility as well).