IMAGING – 2 questions – 5 points total Question 5. requires…
IMAGING – 2 questions – 5 points total Question 5. requires some drawing and/or some calculations. Please do this problem on a separate sheet of paper, then take a picture of your work and submit it in the field below the question.
Read DetailsSURFACE PLASMON RESONANCE – 2 questions – 5 points total Que…
SURFACE PLASMON RESONANCE – 2 questions – 5 points total Question 13. requires some drawing and/or some calculations. Please do this problem on a separate sheet of paper, then take a picture of your work and submit it in the field below the question.
Read Details3. MICROSCOPY a. (2 pt) You want to image two fluorescent pr…
3. MICROSCOPY a. (2 pt) You want to image two fluorescent proteins within the same sample.mEGFP:Excitation max: 488nmEmission max: 507nmmCherry2:Excitation max: 589nmEmission max: 610nmAssume LEDs are used for excitation that precisely match the excitation maxima. Which type of filter would be best to use as an emission filter to detectmCherry2? a. Longpass filter with a 50% cut-off at 520nmb. Shortpass filter with a 50% cut-off at 600nmc. Bandpass filter with 50% cut-offs at 520nm and 660nmd. Bandpass filter with 50% cut-offs at 595nm and 625nm b. (3 pts) Briefly describe the components of a filter cube and their purposes.
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