Upload an image of your answer to this question. Automated E…
Upload an image of your answer to this question. Automated Edman degradation was used to determine the amino acid sequence of the proteins as described in the N-terminal amino acid Sequence determination section of the paper. Using this scheme as a guide, add any key missing O, N, or H atoms along with electron pushing arrows to complete the reaction mechanisms for 1., the addition of the Edman reagent (phenyl isothiocyanate or PITC) to form the PTC derivative, the intermediate cyclic compound, and 2., the breaking of the peptide bond to form the intermediate thiazolinone intermediate. Image Description PITC (Phenyl Isothiocyanate) react with an amino acid residue as part of a peptide chain and produce PTC Derivative. PTC derivative react with an unknown molecule to form cyclic intermediate called PTH The cyclic intermediate reacts to form the thiazolinone intermediate, releasing a peptide with an amino terminus.
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