Using Fluorescence Recovery After Photobleaching (FRAP), you are studying the behavior of a membrane protein in red blood cells. Which FRAP graph would you expect to obtain if your protein is tethered/anchored to junctional complexes in the cortical cytoskeleton?  

There are three exams each worth 75 points.  Together these three exams count for _________ of my grade.

Membrane-associated proteins are embedded in the lipid bilayer through transmembrane alpha helices.

An antiport can be transformed into symport if the orientation of the transporter in the membrane is reversed (i.e. if the portion of the molecule normally exposed to the cytosol faces the outside of the cell instead).

You have identified a novel protein complex that forms a pore across the plasma membrane of a eukaryotic cell. The pore is made of five protein subunits, each of which contributes a membrane-spanning a helix to form the pore. Each a helix has hydrophilic amino acid side chains on one face of the helix and hydrophobic amino acid side chains on the opposite side.What is the likely arrangement of these five a helices in the membrane?

It is acceptable to plagiarize answers to problem set questions.

Cut-and-Run is a technique used to identify genomic DNA sequences bound by a particular protein.

A Positron Emission Tomography (PET) scan visualizes brain activity by imaging energy use. Why does energy use correlate with neural activity?

For each of the following sentences, fill in the blanks with the best word or phrase selected from the provided lists. Not all words or phrases will be used; each word or phrase should be used only once. Affinity chromatographyForward Genetics Gel filtration chromatography GluaseHybridization Ion exchange chromatographyLigaseMultipotent cellsPluripotent cellsPrimary cell cultures Reverse Genetics Reverse Transcriptase Transformed cell linesUnipotent cellsWestern blotting

All membrane transporters carry out active transport.