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Virtual Lab – Microscopy – Wet Mount (Euglena) Here are the…

Virtual Lab – Microscopy – Wet Mount (Euglena) Here are the four steps to creating a wet mount, listed in proper order: Place a Small Drop of Liquid Apply the Cover Slip Adjust and Examine Position the Specimen

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Which disorder typically develops within 3 to 6 months of a…

Which disorder typically develops within 3 to 6 months of a devastating event?

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Edward is in the midst of a major depressive episode. He has…

Edward is in the midst of a major depressive episode. He has just learned that his best friend is going through a divorce and feels responsible for it, even though he had nothing to do with it. Which symptom of depression is this? 

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Stop recording on Screencastify. Click “Export video” in the…

Stop recording on Screencastify. Click “Export video” in the bottom right corner. Choose “Get embed code” and copy the code. In this text box, click the “Insert” drop down, click “embed” and paste the code.   You should now see the video in the textbox and can even play it back to be sure everything went well.   Do not submit the quiz without embedding here!

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Determine the Big-O and the growth rate for the following co…

Determine the Big-O and the growth rate for the following code:     public static int mystery(int[] arr) {         int sum = 0;         int sum2 = 0;         int i = 0;         while (i < arr.length) {           sum += arr[i];           i++;           int j = 0;           while (j < arr.length) {             sum2 += arr[j];             j += arr.length / 2;           }         }         return sum + sum2;     }  

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Write a constructor for HoneyBee. The constructor will take…

Write a constructor for HoneyBee. The constructor will take the name, velocity, and honeyCount and set all instance variables appropriately. Remember that you cannot directly assign the name and velocity, and that HoneyBee is a child class.

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Gel electrophoresis is a technique used in molecular biology…

Gel electrophoresis is a technique used in molecular biology to separate a variety of types of polar molecules by applying electricity using a gel matrix. This method sorts of molecules by size, due to being run through a gel matrix containing pores and separates molecules by charge due to the applied electrical field. The gel matrix is made of agarose, a substance extracted from seaweed. Agarose is neutral in charge; thus, it does not interfere with the separation of sample molecules during gel electrophoresis. When agarose is mixed with an aqueous solution, it makes a semisolid gel medium, which is porous. Much like a sponge, sample molecules in the gel will move about according to their sizes and how they are restricted by the size of the pores. The more concentrated the gel mixture, the smaller the pores will be, which will cause molecules to move slower as they migrate through the gel. To get the molecules to move through these agarose pores, gel electrophoresis requires the application of an electrical field. Positively charged molecules will be attracted to the negatively charged electrode (cathode) of the gel chamber. Negatively charged molecules (such as DNA and RNA) will be attracted to the positively charged electrode (anode) of the gel apparatus. Also, as molecules travel on the gel, smaller molecules travel faster than larger molecules. Over time, the mixture of molecules separates into bands. All of this is accomplished in a gel electrophoresis chamber. This technique works well for separating DNA and RNA because these macromolecules are negatively charged. The phosphate molecules on the backbones of DNA and RNA are highly negative. Therefore, when DNA or RNA samples are loaded into the wells of the agarose gel, they move toward the anode of the electrophoresis chamber. A gel is placed in a buffered solution (TAE buffer) to apply to the electrical field. In an electrical field, electrons (electricity) are able to move more quickly with less resistance. The buffer serves to provide the necessary ions to conduct electricity, but also serves to maintain a stable pH and temperature during gel electrophoresis. When an electrical field is run through an aqueous solution, naturally, water molecules ionize as the molecular movement increases. 2H2O   ->  H3O+ + OH- The increased H+ in solution results in a change of pH and an increase in temperature. To keep the gel from melting during the electrophoresis process, it is essential to use a buffer in the chamber. Loading samples into an agarose gel Use a clean micropipette tip between each sample. Steady hand and slow release of the sample solution into the well. Results Molecules of difference size and charge will separate. Smaller molecules will migrate faster than larger molecules. Positively charged molecules will migrate toward cathode (the negative electrode). Negatively charged molecules will migrate toward anode (the positive electrode). The “thicker” the band, the more molecules are in that spot.

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Virtual Lab – Microscopy – Plant Cells (Onion Epidermal Cell…

Virtual Lab – Microscopy – Plant Cells (Onion Epidermal Cells) Many organelles are not visible in onion cell images primarily due to the following reasons: Size and Resolution Cell Wall and Structure Staining Techniques

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Virtual Lab – Microscopy – Animal Cells (Human Epidermal Cel…

Virtual Lab – Microscopy – Animal Cells (Human Epidermal Cells) What was the general shape of the cheek cell? How is that different from the onion cells you observed from the last simulation?

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Virtual Lab – Label the Parts and Describe the Function of t…

Virtual Lab – Label the Parts and Describe the Function of the Parts of the Microscope Directions: Use the word bank to complete the table below by matching each microscope part to its corresponding number.  Mechanical Stage Rotating Nosepiece Fine Focus Knob Objective lens Coarse Focus Knob Oculars Condenser Stage adjustment knobs Iris Diaphragm Light source       1. [part1] 6. [part6] 2. [part2] 7. [part7] 3. [part3] 8. [part8] 4. [part4] 9. [part9] 5. [part5] 10. [part10]          

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