You are the technical supervisor of the blood bank.  One of your techs suggests that it would be more cost effective to perform antibody screening on patients using commercially available pooled reagent red blood cells rather than a three-cell screen.  The tech reasons that the pooled reagent requires only one gel well for testing versus three wells for the typical screen cells.  This would mean cost savings!  Is it acceptable to use pooled screening cells for patient pre-transfusion testing?  Why or why not?

Polyspecific AHG contains:

You have determined antibody specificity based on ruling out using panel cells that did not react. Explain how you have ruled in suspected antibody(ies) in this case. Is there additional testing that needs to be done to reach a conclusion?

What protocol is put in place to validate Rh testing when high-protein reagents are used, especially when the patient types as an AB-positive?

Of the three following categories of altered D antigen, in which variation of D antigen expression are you more likely to encounter an allo-anti-D?

A type-A person demonstrates a 3+ reactivity with A1 cells (reverse grouping). The forward grouping with anti-A is 4+. Therefore, the possibility of a subgroup is excluded. B cells demonstrate a 4+ reaction. The antibody screen is weakly positive at 37°C, but shows no reactivity at the AHG phase. An antibody panel is performed and anti-M is identified. The patient phenotypes negative for M antigen. How is the ABO discrepancy resolved?

In performing an antibody screen by the solid phase technique, a monolayer of red blood cells is formed at the top of the microplate wells following the addition of indicator cells. This result should be interpreted as:

The process of removing antibody from serum by combining a serum sample with appropriate red blood cells under optimal conditions is called:

What alternative type can be given to an O-positive person when group O blood is not available?

Choose the correct pronouns. Between you and (I, me), we will do a better job than (he, him).