Below are various steps in the cloning process. Which is the…
Below are various steps in the cloning process. Which is the correct order? 1. Transform vector into bacteria. 2. Cut the vector and chromosomal DNA with the same restriction enzyme. 3. Add DNA ligase. 4. Plate on growth media containing an antibiotic such as ampicillin.
Read DetailsThe restriction enzyme EcoRI recognizes the sequence: 5′-G/A…
The restriction enzyme EcoRI recognizes the sequence: 5′-G/AATTC-3′ 3′-CTTAA/G-5′ It cuts between the G and A as noted by the slashes. Following the digestion of chromosomal DNA and vector DNA with EcoRI, let’s suppose that one fragment of chromosomal DNA gets inserted into a vector. No DNA ligase has been added. If both of the sticky ends of the chromosomal DNA fragment are binding to the sticky ends of the vector, what is holding these two pieces of DNA together?
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