A patient with abnormally high iron levels is tested to determine if hemachromatosis, specifically the C282Y mutation in the HFE gene, is the cause. The patient’s DNA is isolated from whole blood and amplified. Amplicons are then cut with Rsa1, and the resulting fragments are separated by gel electrophoresis. The lane containing the patient’s sample has three bands at 240 bp, 140 bp, and 110 bp. Controls were acceptable. Which of the following is the correct interpretation of these results?

A quantitative method was performed 100 times, and results from 99 of the 100 runs were in agreement with each other. This method has 99%:

From the case study in the previous question (#97), If the modified method had been validated, what should have been done with the PT samples? 

The purpose of endonucleases is to:

Which of the following translocations is associated with T-cell lymphoma?

The RNA polymerase holoenzyme consists of which of the following?

The temperature in a refrigerator that is used to store patient specimens and reagents should be checked how often?

A very limited amount of nucleic acid, 500-1500 bp in size, needs to be analyzed in a short time (same day) with the results available immediately. Which of the following electrophoresis procedures will satisfy those conditions?

Human chromosomes are described as which of the following with regard to the location of the centromere?

A woman needs an organ transplant, and her husband volunteers to donate his organ. In pretransplantation testing, the woman’s serum was mixed in vitro with lymphocytes from her husband. After a few hours, complement and trypan blue were added to the culture well. The cells were observed under the microscope and determined to be viable. What is the interpretation of this assay?