2007 Information: On 12/31/2006, USF had a Pension Liability of $3,500,000, which was comprised of a $23,000,000 PBO and $19,500,000 of Plan Assets.  During 2007, USF contributed $4,000,000 to their pension plan, and paid out $5,000,000 in benefits.  They expected to earn a 10% return on their Plan Assets, but they actually earned 4%.  The actuary has told them to use a 5% settlement rate for interest for 2007, and has estimated a $3,000,000 current service cost. There was a $2,100,000 Net Loss in their AOCI account related to prior year actual returns being less than expected.  At the end of the year their actuary revises the discount rate down, resulting in a loss of $1,000,000. The average remaining service life of the current employees is 15 years. 2008 Information: On January 1, USF decides to amend its pension plan in the current year, which results in an increase in the PBO of $3,000,000 related to prior service costs.  The average remaining service of the affected employees is 5 years.  USF again expects to earn a 10% return on their Plan Assets, and they assume a 4% settlement rate for interest in 2008.  Plan Assets earn an actual return of 6%, and current service costs totaled $4,000,000.  USF contributed $4,000,000 to the plan assets, and paid $3,000,000 in benefits to plan participants. At the end of the year their actuary revises the discount rate down again, resulting in another loss of $1,500,000. The average remaining service life of the current employees is now 10 years. What is the Plan Asset balance at the end of 2008?

Dr. Jones is doing research on an endangered species of leopard living wild in the African savannah. This method is a bit more dangerous than some methods and does not give him much control over his subjects. Which method of observation is he using?

A circular collimator(diaphragm) produces a round beam that is _____ inch (es) in diameter.

A small(short) object-film distance will increase magnification of the tooth image.

The definition of vertical angulation is:

Which type of genomics file is most efficient for obtaining all mutations that lead to changes in amino acid sequences?

Which of the following is considered the oldest form of biotechnology that was developed about 6000 BC?

A 78-year-old who has been admitted to the hospital with dehydration is confused and incontinent of urine. Which nursing action will be best to include in the plan of care?

Your goal is clone your favorite gene NRG1 using restriction digestion with type II restriction enzymes, followed by ligation in a pET plasmid, and transformation in E. coli competent cells.  The objective is to clone NRG1 between the NcoI and XhoI of the pET22b. This will allow NRG1 protein to be in frame with the pelB leader peptide and be transported to the E. coli periplasm (secretion pathways are cool), where NRG1 can fold properly. NRG1 can be amplified by PCR from cDNA of neuronal cells. How will you make sure that NRG1 will be ligated to the NcoI and XhoI sites of pET22b? (Alternatively, you can outline the steps necessary to clone a gene). 

Refer to the table below to answer the following questions.  You obtained some RNA sequencing data. Below is the 5′- sequence of a disease-associated transcript frame that should produce a protein.  GAGAUCCUAUGGAGGACACAGUAUGUGAGAGUAGCCA.  What is the partial amino acid sequence of the protein?