9. Choose the answer that has these events of protein synthesis (translation) in the proper sequence. 1. A new aminoacyl-tRNA binds to the A site (codon recognition)2. A peptide bond forms between the new amino acid and a growing polypeptide chain in A site (peptide bond formation)3. Empty tRNA leaves the P site, and the P site remains vacant 4. A small ribosomal subunit binds with mRNA, the large ribosomal subunit binds, and the initiator tRNA carrying met binds to the P site (initiation) 5. tRNA carrying the growing polypeptide translocates from A site to the P site (translocation) 

8. In order to function as an effective cloning vector, plasmids MUST contain all following, EXCEPT? 

5. How would you name the 1st restriction enzyme isolated from Strain R of the bacterial species E. coli?

1. For a couple of decades, biologists knew that the nucleus contained DNA and proteins. The prevailing opinion was that the genetic material was proteins, and not DNA. The reason for this belief was that proteins are more complex than DNA. What was the basis of this thinking?

6. During DNA Replication, The Leading Strand template strand is read in the _______ direction, and The Lagging Strand template strand is read in the _______ direction?

9. Place the steps in a cycle of PCR (Polymerase Chain Reaction) in the correct order.    1.Annealing—Cool mix to allow primers to form hydrogen bonds with ends of target sequence 2.Extension—DNA polymerase adds free nucleotides to the 3′ end of each primer 3.Denaturation—Heat briefly to near boiling temp to separate DNA strands

10.  Why is a PCR (Polymerase Chain Reaction) mixture incubated to near boiling (~ 98°C)?

4. Which of the following components does NOT form part of the transcription complex at a eukaryotic promoter?

3. If the result of the Alfred Hershey and Martha Chase experiment had been that radioactive sulfur(35S) was found inside the cells instead of radioactive phosphorus (32P), what could have been concluded?

8. A particular diploid plant species has 48 chromosomes, or two sets. A mutation occurs and gametes with 48 chromosomes are produced. If self-fertilization occurs, the gametes will have _____ set(s) of chromosomes. This is called ________?