A tech is working with a small Gram-negative coccobacillus that may be Francisella tularensis. Which of the following tests could the tech use to rule out this organism?

A tech is working with a small, Gram-negative coccobacillus that may be Brucella spp. Which of the following tests could the tech use to rule out this organism?

This species is the most commonly reported laboratory-acquired bacterial infection and must be handled in class II or higher biological safety cabinet. They are small gram-negative coccobacilli that are strict aerobes and will grow on Sheep Blood Agar and Chocolate agar. They are nonmotile, catalase, urease, and nitrate positive and most strains are oxidase positive. From the choices below, please select the organism matching the microscopic and biochemical reactions listed:  

The picture below shows 3 sets of innoculated OF glucose test medium. 1) Which set (A, B, or C) would be indicative of an “Oxidizer”. 2) Which organism might this be (just give me any organism that oxidizes glucose)?    

The granular, wrinkled, yellow/white-pigmented colonies shown in the photograph were recovered on chocolate agar after 48 hours of incubation from a respiratory specimen collected from a patient with cystic fibrosis. Note in the lower composite photograph that colonies on the surface of blood agar are flat, granular, and only slightly wrinkled. On MacConkey agar the colonies lack lactose fermentation and lack pyocyanin production. The spot oxidase test is positive, and nitrates are reduced. With these observations, select the presumptive identification of this isolate. The answer is in the morphology.  

A patient with cystic fibrosis is admitted with a fulminant lung infection. A sputum culture grows a non-lactose fermenting, oxidase positive, Gram-negative bacilli. It is pyocyanin negative, nitrate reduction variable, and it is arginine dihydrolase negative.

A tech is working with a small, Gram-negative coccobacillus that may be Brucella spp. Which of the following tests could the tech use to rule out this organism?

The granular, wrinkled, yellow/white-pigmented colonies shown in the photograph were recovered on chocolate agar after 48 hours of incubation from a respiratory specimen collected from a patient with cystic fibrosis. Note in the lower composite photograph that colonies on the surface of blood agar are flat, granular, and only slightly wrinkled. On MacConkey agar the colonies lack lactose fermentation and lack pyocyanin production. The spot oxidase test is positive, and nitrates are reduced. With these observations, select the presumptive identification of this isolate. The answer is in the morphology.  

The bacterial species growing on the surface of the MacConkey agar plate shown in this photograph, a coccobacillary non-fermenter and a common cause of nosocomial hospital-acquired pneumonia, can be presumptively identified as:

Pseudomonas aeruginosa produces pyocyanin (bright bluish pigment on Mueller-Hinton agar)?