A twofold serial dilution is performed in 1.0 ml amounts fro…
A twofold serial dilution is performed in 1.0 ml amounts from a MH broth containing ampicillin. Tubes 1 through 11 are incubated overnight at 35 degrees C. Tubes 6 through 11 show visible turbidity while tubes 1 through 5 show no signs of growth. Tubes 1 through 5 are plated on blood agar and incubated overnight. Plates 1 through 4 show no growth, while several colonies are observed on plate 5. The MIC would be determined from which tube?
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