You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzymes should you use?  

You design a PCR primer to a target sequence with an EcoRI overhang. The overhang bases (not the sequence-specific bases)

When two sticky ends join, they make ________ between the bases, and ________ between the DNA backbones.

What is the Tm of the primer CGTAT?

Which molecule allows for real-time visualization of DNA during gel electrophoresis?

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “B” represent?

The purpose of screening a library is to

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “A” represent?

Which technique can detect pathogens in blood samples by using RNA polymerase to synthesize mRNA, which can be detected with a probe?

You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzymes should you use?