A mutation (GCC) was introduced into the protein-coding gene… A mutation (GCC) was introduced into the protein-coding gene below. What primers were used to introduce this mutation? 5’-AACGAGAATGGGCGCCGCGACAGATTTGAC-3’ Read Details
Which technique allows one to screen bacterial strains for m… Which technique allows one to screen bacterial strains for mutations, or to track down the origin of a pathogen in food? Read Details
Which of the following is FALSE regarding Taq and Pfu DNA po… Which of the following is FALSE regarding Taq and Pfu DNA polymerases? Read Details
Which technique would be best for single cell DNA sequencing… Which technique would be best for single cell DNA sequencing? Read Details
To introduce a His-tag onto a protein at the C-terminus, you… To introduce a His-tag onto a protein at the C-terminus, you would need to Read Details
All of the following were essential to the development of PC… All of the following were essential to the development of PCR EXCEPT Read Details
You design two primers with melting temperatures of 50 °C an… You design two primers with melting temperatures of 50 °C and 60 °C. If both of these primers were to be used together in PCR, what should the ideal annealing temperature be? Read Details
The gel below analyzes a family via RFLP. Which of the follo… The gel below analyzes a family via RFLP. Which of the following children are recombinant at the A locus based on the gel? Read Details
When performing RT-PCR which of the following should be used… When performing RT-PCR which of the following should be used as the reverse primer? Read Details
