You insert a gene into the vector below using the NdeI and SstI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

You wish to use Sanger sequencing to sequence a 1000 bp insert that is in a vector. Which of the following is TRUE?

Cosmids are useful because they can

EtBr is often used to visualize DNA on a gel for all of the following reasons EXCEPT

Which of the following will occur if the annealing temperature in PCR is 5 °C above the primer Tm?

Some restriction enzymes cut incorrectly in the wrong buffer. This is called

Which lane below represents a digestion with PstI and SmaI?

All of the following are used as vectors for human gene therapy EXCEPT

To purify DNA from an agarose gel you would perform a(n)

You use Sanger sequencing on a 1000 bp insert, but after ~500 bp the data becomes difficult to interpret. What is the next step?