A mutation (TGA) was introduced into the protein-coding gene below. What primers were used to introduce this mutation? 5’-AACGAGAATGGCATGAATGACAGATTTGAC-3’ 

In touchdown PCR,

In the gel below, the DNA in Lane 5 is approximately

In TA cloning,

A DNA microarray is typically used for all of the following EXCEPT

You digest a ~2000 bp plasmid with EcoRI, and run a linear marker. The digestion at various time points is shown below. Which of the following does “D” represent?

The most commonly-used method to detect DNA on a gel is

You are working with a vector that has both an ampicillin and kanamycin resistance gene. If you insert your gene into the origin of replication, the colonies will

You wish to determine which amino acids of a very large enzyme are involved in the active site but are unsure of where to begin. One practical approach would be to

All of the following explain why PCR usually limited to ~30 cycles EXCEPT