Which type of genomics file is most efficient for obtaining all mutations that lead to changes in amino acid sequences?

Which of the following is considered the oldest form of biotechnology that was developed about 6000 BC?

A 78-year-old who has been admitted to the hospital with dehydration is confused and incontinent of urine. Which nursing action will be best to include in the plan of care?

Your goal is clone your favorite gene NRG1 using restriction digestion with type II restriction enzymes, followed by ligation in a pET plasmid, and transformation in E. coli competent cells.  The objective is to clone NRG1 between the NcoI and XhoI of the pET22b. This will allow NRG1 protein to be in frame with the pelB leader peptide and be transported to the E. coli periplasm (secretion pathways are cool), where NRG1 can fold properly. NRG1 can be amplified by PCR from cDNA of neuronal cells. How will you make sure that NRG1 will be ligated to the NcoI and XhoI sites of pET22b? (Alternatively, you can outline the steps necessary to clone a gene). 

Refer to the table below to answer the following questions.  You obtained some RNA sequencing data. Below is the 5′- sequence of a disease-associated transcript frame that should produce a protein.  GAGAUCCUAUGGAGGACACAGUAUGUGAGAGUAGCCA.  What is the partial amino acid sequence of the protein? 

Still keeping with your cloning of NRG1. Something terrible happening. You find that NRG1 contains an NcoI cut site right in the middle of the gene. What other methods could you use to clone NRG1 between NcoI and XhoI? Explain your procedure. 

The nurse is caring for a client with early encephalopathy due to cirrhosis of the liver. Which factors may contribute to increased encephalopathy for which the nurse would assess? Select all that apply.

The nurse is caring for a patient who came into the emergency department with shortness of breath. The physician ordered an EKG and the nurse notes a prolonged QT interval. What medication on the patient home medication list will the nurse address with the physician?  

What occurs in sexual reproduction that leads to the incredible variation of offspring?

In Mendel’s experiments, the first generation of plants that he got, from crossing parental generation plants, exhibited only  _______ traits, while the next generation of plants, which resulted from self-pollination of plants, exhibited both  _______ and _______ traits, in a 3:1 ratio