Rick’s three-year-old son, Brian, is particularly fearful.  Give Rick four suggestions for helping Brian deal with his fears.

The fear of being away from familiar people and places is known as anxiety.

(12 pts) The chemical shift is one of the most important experimental NMR parameters which becomes readily available at a very early stage of a protein NMR study. Amino acid chemical shifts are determined by the corresponding covalent structure (identity of amino acid; e.g. Gly vs. Ala or atom in an aromatic sidechain vs. atom in an aliphatic sidechain), the neighboring amino acids (flanking in the primary sequence), interactions with other amino acids through space (e.g. spatial vicinity in a folded protein). Shown below are the “fingerprint” 2D 1H-15N HSQC spectra of a protein acquired under two conditions (A and B).     a) The resonances of which exactly atoms are correlated in a 2D 1H-15N HSQC experiment? (4 pts)   b) Which of the two conditions (A and B) corresponds to non-native conditions (9M urea) and which to native conditions (0M urea)? Justify your answer (8 pts) 

(2 pts) Where on the below diffraction image would you expect to find the reflections that correspond to the highest resolution data? 

gabapentin question, pregabalin question, 

(4 pts) Would you expect to see more detail in a crystal structure that has a resolution of 1 Angstrom or 3 Angstrom? Why? 

(3 pts) What does the B-factor measure? 

79Ft with chronic cancer-related pain is receiving fentanyl 50 mcg IV every 2 hours PRN for severe pain. Due to a change in clinical status, the provider decides to switch to IV hydromorphone. Using equianalgesic dosing, what is the most correct dose of hydromorphone? IV fentanyl 100 mcg ≈ IV morphine 10 mg  

23F is following up with the neurology clinic after her hospitalization for IVH into the temporal space. She is being switched to phenytoin for her AED. She asks why she was started on levetiracetam inpatient if the ultimate plan was to transition to phenytoin. The RN’s best explanation is:

(4 pts) Using the crystallization growth chart below, please explain exactly where on this chart you would want your crystals to first form in order to obtain an optimal drop of crystals. Then please explain why you would want them to start growing in the spot you chose.