Which of the following will occur if the annealing temperature in PCR is 5 °C above the primer Tm?

Some restriction enzymes cut incorrectly in the wrong buffer. This is called

Which lane below represents a digestion with PstI and SmaI?

All of the following are used as vectors for human gene therapy EXCEPT

To purify DNA from an agarose gel you would perform a(n)

You use Sanger sequencing on a 1000 bp insert, but after ~500 bp the data becomes difficult to interpret. What is the next step?

Which of the following will occur if the annealing temperature in PCR is 5 °C below the primer Tm?

In RAPD PCR,

Who discovered Thermus aquaticus, the bacteria from Yellowstone which was the source of Taq polymerase?

You want to design a protein that contains an EcoRI cut site on the N-terminus, and a BamHI site at the C-terminus. What should the primers look like?