Why do DNA gels not need a stacking gel, which are used in SDS-PAGE?

Which of the following is TRUE regarding bacteriophage lambda vectors?

The image below is illustrating

Which of the following is FALSE about restriction enzymes?

Klenow fragment is useful for

RNA-seq

You insert a gene into the vector below using the KpnI and EcoRI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

In multiplex PCR,

In qPCR, two different samples can be compared to determine relative amounts by using

You perform PCR with two primers with melting temperatures of 50 °C and 60 °C. To ensure proper binding, you use an annealing temperature of 30 °C. Which is TRUE?