You wish to amplify a piece of genomic DNA (it is not protei… You wish to amplify a piece of genomic DNA (it is not protein-coding) and ligate it into a vector using EcoRI and BamHI. What should the primers look like? Read Details
Which of the following enzymes can be used to join together… Which of the following enzymes can be used to join together two fragments of DNA? Read Details
Bacteria that take up DNA from the environment are said to b… Bacteria that take up DNA from the environment are said to be Read Details
A primer has a Tm of 50 °C, but you attach a restriction enz… A primer has a Tm of 50 °C, but you attach a restriction enzyme cut site which increases it to 55 °C. Which of the following is TRUE? Read Details
Which of the following transformation techniques provides a… Which of the following transformation techniques provides a time constant, which indicates if transformation was successful? Read Details
You wish to optimize a PCR reaction, and perform the experim… You wish to optimize a PCR reaction, and perform the experiment at several different temperatures (from ~40-60 °C). Which step of PCR is was modified? Read Details
Which technique involves linking primers directly to beads t… Which technique involves linking primers directly to beads to allow for easy retrieval, analysis, and to reduce contamination? Read Details
In the pedigree below, which represents the dot blot you’d e… In the pedigree below, which represents the dot blot you’d expect of the child? Read Details
Three DNA samples undergo VNTR analysis, as shown below. Arr… Three DNA samples undergo VNTR analysis, as shown below. Arrows denote digestion sites. Which of these three individuals is represented in Lane 2 on the gel to the right? Read Details
