Below is a fragment of DNA sequence encoding a gene. Some of…
Below is a fragment of DNA sequence encoding a gene. Some of the important genetic elements are underlined and marked in red as shown below. Please design an anti-sense regulatory sRNA that can down-regulate the translation of this gene. The sRNA sequence should be 20 nt long. Please write down the sRNA sequence from 5’-3’. [2 pts] 5′-TAGTTGACATACATAATTCGTGACATTATAATGACTTTACAAATACAT ACAGGAGGTTCTGAATGGCGCAACACGATGAAGCTCAACAAAATGCT TTTTATCAAGTCTTAAATATGCCTAACTTAAATGCTGATCAACGCAATG GTTTTATCCAAAGCCTTAAAGATGATCCAAGCCAAAGTGCTAACGTTT TAGGTGAAGCTCAAAAACTTAATGACTCTCAAGCTCCAAAAGCTGATG CGCAACAAAATAACTTCAACAAAGATCAACAAAGCGCCTTCTATGAA ATCTTGAACATGCCTAACTTAAACGAAGCGCAACGTAACGGCTTCATT CAAAGTCTTAAAGACGACCCAAGCTAACGTGACATTTCGCCTCCGGT AGGAGGCGTTTTTTTTGCTA -3’
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