During SDS-PAGE electrophoresis, proteins are separated based on size only.

A supercoiled plasmid will run faster compared to its linearized form, because it is more compact.

Protein samples from three patients (1,2 and 3) were separated by SDS-PAGE and subjected to immunoblot analysis.  The blot was incubated with antibodies specific for B-actin and VEGF proteins. Why was the blot probed with antibodies specific for B-actin? Pick all correct answers for complete credit.

The following plasmid was inserted into a bacterial cell and its presence was confirmed.  However, the plasmid failed to replicate inside the cell.  Which of the following answers could best explain the reason for this failure?  

The following steps of a PCR are out of order.  What is the correct order of events that need to occur to amplify DNA?

A cell that has received the following plasmid could not survive in the presence of the antibiotic, Ampicillin.  What could be a possible explanation for this?

What does “X” represent in the following picture?  

Compare Samples A and B. Which sample has pure RNA?

10. How does the Wife of Bath describe her physical appearance?

5. What did the Wife of Bath do when her fourth husband was unfaithful?