Which of these are not steps in a PCR reaction? (Select all that apply)

Mass spectrometry identifies molecules based on what?

Based on what you know about CRISPR-Cas9, mention a real-life scenario where it would be beneficial to use this technology. Why would it be useful? (Be brief, but specific)

The activity of an enzyme is analyzed and it is found to have a Km value of 2.551 mM and a Vmax value of 0.174 mM/min. An inhibitor is introduced to the system, and the activity of the enzyme is re-analyzed. In the presence of this inhibitor, the enzyme is found to have a KM value of 2.551 mM and a Vmax value of 0.133 mM/min.  Based on the observed change in the enzyme’s activity, what kind of inhibitor do you believe is present in the system and why?

During PCR in Next Gen Sequencing, one pair of primers is used to amplify all different sequences. 

In your own words, briefly describe the CRISPR-Cas9 technology. 

Part of good lab practice is to identify the safety equipment around you and to label all samples with content, potential hazards, date and your initials.

Transcription occurs in the [answer1] while translation occurs in the [answer2] in organelles called [answer3].

A researcher is performing PCR to amplify a sample of DNA. Unfortunately, he forgot to add the DNA primer prior to starting the experiment. Briefly describe the results you expect he will observe and explain why you believe he will obtain these results. 

What is the template of a Polymerase Chain Reaction (PCR)?