9. Place the steps in a cycle of PCR (Polymerase Chain Reaction) in the correct order.    1.Annealing—Cool mix to allow primers to form hydrogen bonds with ends of target sequence 2.Extension—DNA polymerase adds free nucleotides to the 3′ end of each primer 3.Denaturation—Heat briefly to near boiling temp to separate DNA strands

10.  Why is a PCR (Polymerase Chain Reaction) mixture incubated to near boiling (~ 98°C)?

4. Which of the following components does NOT form part of the transcription complex at a eukaryotic promoter?

3. If the result of the Alfred Hershey and Martha Chase experiment had been that radioactive sulfur(35S) was found inside the cells instead of radioactive phosphorus (32P), what could have been concluded?

8. A particular diploid plant species has 48 chromosomes, or two sets. A mutation occurs and gametes with 48 chromosomes are produced. If self-fertilization occurs, the gametes will have _____ set(s) of chromosomes. This is called ________?  

1. Which of the following molecules bridges DNA to protein? 

5.  In the late 1950s, Meselson and Stahl grew bacteria in a medium containing heavy nitrogen (15N) and then transferred them to a medium containing lighter nitrogen (14N). Which of the results in figure below would be expected after one round of DNA replication in the presence of 14N?  

8. Roses exhibit incomplete dominance. If 2 pink heterozygous roses are allowed to breed, what ratio of phenotypes is expected in the offspring?

3. Achondroplasia, a common form of dwarfism, is caused by a Dominant allele.  If 2 Heterozygotes for this trait were to mate, what % of their offspring will be normal height? 

7. To say a mammalian gene is sex-linked typically implies that it?