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Vesicular transport across the endomembrane system can occur…

Vesicular transport across the endomembrane system can occur in coated vesicles covered by: (select all that apply)

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SLE (Lupus) involves an autoimmune-induced inflammation. Joi…

SLE (Lupus) involves an autoimmune-induced inflammation. Joint pain is the most common symptom and results from inflammation around the joint. The inflammatory process can increase proteolytic activity (breakdown of proteins)  in the ECM. In a specific case of SLE, lab tests suggest that water retention drops dramatically in cartilages. This can result in joint damage and pain. A. Based on this information, degradation of which protein will be most affected in SLE? (2 points) B. Which of the following hydropathy plots will better represent the affected protein? Briefly explain your answer (4 points)

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In one experiment, you coat a petri dish with fibronectin an…

In one experiment, you coat a petri dish with fibronectin and proteoglycan and culture cells on a petri dish. Cells adhere to the dish. In another set of experiments (Experiments 1-4), you repeat the previous procedure but this time add one synthetic peptide to each of the culture dishes as the cells are added. A. What are the outcomes of experiments 1 to 4? Hint: do cells adhere or not in each experiment? (4 points) B. Explain how you determined if cells adhered or not in experiments 1 to 4 (4 points)  

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Autoradiography is a technique that allows to track the inco…

Autoradiography is a technique that allows to track the incorporation of radiolabeled compounds.  In which part(s) of the cell would you expect radiolabeled Leucine to be incorporated?

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Bonus question: You identify a new enzyme called “signalase”…

Bonus question: You identify a new enzyme called “signalase” that removes the signal peptide of any protein that contains it. You expose the following proteins to the action of signalase. Which ones  would be affected by this treatment?  Select all that apply

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According to the assigned reading, the ability to feel “lump…

According to the assigned reading, the ability to feel “lumps” during a breast exam is associated with

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The unfolded protein response (UPR): Is triggered when ther…

The unfolded protein response (UPR): Is triggered when there are too many misfolded proteins in the ER. Is inhibited by BiP molecules. Occurs in the cytoplasm Involves the synthesis of more chaperones in the ER. Inhibits enzymes involved in protein synthesis.

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A cell that is actively transporting proteins is exposed to…

A cell that is actively transporting proteins is exposed to COPII-coat protein antibodies. What happens?   1. Budding of vesicles from the ER to Golgi is blocked  2. Budding of vesicles from Golgi to ER is blocked  3. Budding of vesicles from Golgi to lysosomes is blocked  4. The process of quality control would be affected  5. The process of secretion of components of the ECM would be affected

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How do tetanus and botulism toxins cause muscle paralysis?

How do tetanus and botulism toxins cause muscle paralysis?

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A VSVG-GFP gene has been mutated to become a temperature sen…

A VSVG-GFP gene has been mutated to become a temperature sensitive mutant. It is also associated with two types of Temperatures; namely, 32 °C and 40 °C. Which one of the following better describes what happens when the cells are incubated at 40 °C? Hint: assume you are conducting biosynthetic pathway experiments.

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