A first morning sputum is received for culture of mycobacter…
A first morning sputum is received for culture of mycobacteria. It is digested and concentrated by the N-acetyl-L-cysteine alkali method. Two Lowenstein-Jensen slants are incubated in the dark at 35° C with 5-10% CO2. The smears reveal acid-fast bacilli, and after 7 days, no growth appears on the slants. The best explanation is:
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