You are interested in receptor protein-tyrosine kinases and you are purifying a novel phosphotyrosine-binding protein (NPBP-1).  In order to characterize this protein you need to separate it from three other proteins (X, Y, and Z) that are still present in your partially purified material.  The proteins in your preparation have the following properties:      binds to phosphotyrosine Size (kDa) pI protein X yes 85 7.2 protein Y yes 29 2.9 protein Z no 31 7.4 NPBP-1 yes 28 7.5   A. What type of separation technique can be used to separate protein NPBP-1 from protein X? [1] B. What type of separation technique can be used to separate protein NPBP-1 from protein Y? [2] C. What type of separation technique can be used to separate protein NPBP-1 from protein Z? [3]

Lynn Margulis’ Theory of Endosymbiosis is an explanation for the origin of

How many amino acid residues are present in the peptide shown above? [1] Which is the N-terminal amino acid in the peptide shown above? [2] What is the three-letter symbol for the C-terminal amino acid in the peptide shown above? [3]

In two homologous proteins, which residue is most likely to replace a Glu residue as a conservative substitution?

A phylogenetic tree depicts ___________ of proteins.

[1] are involved in the nonselective degradation of extracellular and membrane proteins .   ATP-dependent degradation of ubiquitinated proteins  is mediated by [2].

Employers are required to record (on the OSHA log) every injury that occurs at work.

Full compliance with OSHA noise standards will completely eliminate hearing loss in the workplace.

Noise is unwanted sound that can be irritating and damaging.

For recordkeeping, OSHA counts calendar days rather than missed scheduled work days because it is a better measure of severity (e.g., a better measure of the impact of the injury).