You wish to determine which amino acids of a very large enzyme are involved in the active site but are unsure of where to begin. One practical approach would be to

All of the following explain why PCR usually limited to ~30 cycles EXCEPT

Which of the following PCR techniques can be used to analyze gene expression?

You wish to amplify a piece of genomic DNA (it is not protein-coding) and ligate it into a vector using EcoRI and BamHI. What should the primers look like?

Which of the following enzymes can be used to join together two fragments of DNA?

Bacteria that take up DNA from the environment are said to be

A primer has a Tm of 50 °C, but you attach a restriction enzyme cut site which increases it to 55 °C. Which of the following is TRUE?

Which of the following transformation techniques provides a time constant, which indicates if transformation was successful?

You wish to optimize a PCR reaction, and perform the experiment at several different temperatures (from ~40-60 °C). Which step of PCR is was modified?

Which technique involves linking primers directly to beads to allow for easy retrieval, analysis, and to reduce contamination?