In TOPO TA cloning,

Which of the following describes a standard PCR reaction, in order?

Which sequencing method involves detecting the nucleotide directly, without halting synthesis or digesting fragments?

If the annealing temperature in PCR is 5 °C below the Tm of the primers,

You are interested in performing blue and white screening. You plate cells containing a vector with no insert in the presence of ampicillin and X-gal (no IPTG). Which of the following would you expect to observe?

To verify the sequence of an insert in a vector with Sanger sequencing, you must use

You perform Sanger sequencing on a plasmid which came from an E. coli colony. At one position you find two different overlapping peaks at 50% of the height of surrounding peaks, which are all identical to one another. What can you conclude from this?

In ligation independent cloning,

What primers would you design to amplify the following sequence of DNA? 5’-TTGCGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTAGAGTC-3’

100 micrograms/ml of DNA would give an A260 of