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Which of the following is true regarding blunt end digestion…

Which of the following is true regarding blunt end digestion and subsequent ligation?

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What must a plasmid contain to ensure proper replication by…

What must a plasmid contain to ensure proper replication by DNA polymerase?

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Which technique was used to capture Taq-treated DNA for the…

Which technique was used to capture Taq-treated DNA for the sequencing of the human genome?

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In the gel below, the DNA in Lane 3 is approximately

In the gel below, the DNA in Lane 3 is approximately

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A sequencing gel has two clearly defined lanes for G and C,…

A sequencing gel has two clearly defined lanes for G and C, and then a purine and pyrimidine lane. This is likely

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You wish to insert a gene into the vector below, grow the ce…

You wish to insert a gene into the vector below, grow the cells on ampicillin, and select colonies with blue/white screening. Which restriction enzymes should you use?  

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You design a PCR primer to a target sequence with an EcoRI o…

You design a PCR primer to a target sequence with an EcoRI overhang. The overhang bases (not the sequence-specific bases)

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When two sticky ends join, they make ________ between the ba…

When two sticky ends join, they make ________ between the bases, and ________ between the DNA backbones.

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What is the Tm of the primer CGTAT?

What is the Tm of the primer CGTAT?

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Which molecule allows for real-time visualization of DNA dur…

Which molecule allows for real-time visualization of DNA during gel electrophoresis?

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