In the below diagram of the gene locus, available oligonucle…
In the below diagram of the gene locus, available oligonucleotide (ssDNA) primers are depicted as numbered arrows, with the beginning of the arrow representing the 5′ end and the tip of the arrowhead representing the 3′ end. The bracketed lines indicate base pair (bp) distances between segments of the locus. Which combination of primers, used in a PCR reaction, will produce an amplicon of 550 bp?
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