You insert a gene into the vector below using the EcoRI and … You insert a gene into the vector below using the EcoRI and SphI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE? Read Details
Which of the following can be used to inhibit RNase activity… Which of the following can be used to inhibit RNase activity? Read Details
What primers would you design to amplify the following seque… What primers would you design to amplify the following sequence of DNA? 5’-ATCCGTTGAGCAGTTGAGTACTTAGGCCTGCAGTGTCGGATC-3’ Read Details
Which of the following is FALSE regarding the screening of a… Which of the following is FALSE regarding the screening of a gene library? Read Details
You perform Sanger sequencing on an E. coli colony from a pl… You perform Sanger sequencing on an E. coli colony from a plate, and actually see two sequences as shown below. What can you conclude from this? …GTGCACCGC… …GTGCTCCGC… Read Details
A mutation (TGA) was introduced into the protein-coding gene… A mutation (TGA) was introduced into the protein-coding gene below. What primers were used to introduce this mutation? 5’-AACGAGAATGGCATGAATGACAGATTTGAC-3’ Read Details
In the gel below, the DNA in Lane 5 is approximately In the gel below, the DNA in Lane 5 is approximately Read Details
