Isothermal amplification, which can detect pathogens at room temperature, uses

You run a PCR product on a gel and see multiple nonspecific bands, and smearing. Which of the following is NOT a likely cause?

You insert a gene into the vector below using the HindIII and PstI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

Which of the following techniques involves using a primer to sequence a fragment of DNA and then using this new sequence as a primer to sequence the next stretch of DNA?

You insert a gene into the vector below using the NdeI and SstI cut sites, grow the cells on ampicillin, and perform blue/white screening. Which is TRUE?

You wish to use Sanger sequencing to sequence a 1000 bp insert that is in a vector. Which of the following is TRUE?

Cosmids are useful because they can

EtBr is often used to visualize DNA on a gel for all of the following reasons EXCEPT

Which of the following will occur if the annealing temperature in PCR is 5 °C above the primer Tm?

Some restriction enzymes cut incorrectly in the wrong buffer. This is called