Janthinobacterium lividum is a Gram negative bacterium that…
Janthinobacterium lividum is a Gram negative bacterium that produces a dark violet compound called violacein that possess antiviral, antibacterial, and antifungal properties. You are interested in finding gene(s) responsible for the production of violacein. You transform a culture with a plasmid containing a transposon and an ampicillin resistance gene (bla). Realizing your transformation efficiency will be low, how might you identify bacteria that have been successfully transformed?
Read DetailsConsider a population of E. coli mutants that have a defect…
Consider a population of E. coli mutants that have a defect in their lacI gene, resulting in the production of a repressor protein that binds to the operator of the lac operon, but does not bind to lactose itself. If you placed these cells in a growth media containing lactose, what would happen?
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