If a Brain Heart Infusion (BHI) agar tube was inoculated with an aerotolerant anaerobe, the bacteria would grow _____.

Serial ten-fold dilutions are made from a broth culture of Bacillus subtilis in order to determine the number of bacteria in the culture.  Following the dilution series, 0.1 ml of a 1:100,000 (1 x 10-5) dilution is plated, the plate is incubated, and 87 colonies are counted.  Calculate the number of bacteria in the original Bacillus subtilis culture.  Don’t forget units! Make sure your answer reflects proper scientific notation! Use the ^ to denote an exponent, i.e. 1 x 10-5 would be written as 1 x 10^-5. Note: Don’t forget the units. No points without units!

In the Gram staining procedure, Gram negative organisms stain _______________________.

A new bacterial pathogen has been discovered which can cross the blood-brain barrier and cause bacterial meningitis. What temperature classification would you assign to this bacterium?

Which of the following bacteria is an obligate anaerobe?

Give the full name of the media MSA

What color would E. coli be while growing on an EMB plate?

Beta hemolysis on the blood agar plate is indicative of ________________ hemolysis of RBC’s. (Pick from: complete, partial, no)

This is an EMB agar plate. Give an example of a bacterium growing in sector A.

Serial ten-fold dilutions are made from a broth culture of Bacillus subtilis in order to determine the number of bacteria in the culture.  Following the dilution series, 0.1 ml of a 1:10,000 (1 x 10-4) dilution is plated, the plate is incubated, and 110 colonies are counted.  Calculate the number of bacteria in the original Bacillus subtilis culture. Don’t forget units! Make sure your answer reflects proper scientific notation! Use the ^ to denote an exponent, i.e. 1 x 10-4 would be written as 1 x 10^-4. Note: Don’t forget the units. No points without units!